Buy Plant Tissue Culture Media Online – High-Quality Media for Micropropagation, Research, and Commercial Production

Plant tissue culture media are the foundation of modern plant biotechnology, enabling rapid, disease-free, and large-scale propagation of elite plant varieties. When you buy plant tissue culture media online, you gain access to precise, high-purity, and reproducible formulations that support every stage of in vitro plant growth—from callus induction to rooting and acclimatization. Our buy plant tissue culture media online in India service ensures you receive laboratory-grade, quality-assured media with comprehensive documentation, reliable supply, and prompt delivery.

What is Plant Tissue Culture Media?

Plant tissue culture media are specially formulated blends of inorganic nutrients, organic supplements, vitamins, plant growth regulators, and gelling agents that provide the essential environment for the aseptic growth and development of plant cells, tissues, or organs in vitro. These media are the backbone of plant micropropagation, genetic engineering, germplasm conservation, and research in plant developmental biology.

Key Components of Plant Tissue Culture Media

• Macronutrients: Nitrogen, phosphorus, potassium, calcium, magnesium, sulfur (as salts like NH₄NO₃, KNO₃, CaCl₂, MgSO₄, KH₂PO₄)

• Micronutrients: Iron, manganese, zinc, copper, boron, molybdenum, cobalt, iodine

• Vitamins: Thiamine (B1), pyridoxine (B6), nicotinic acid (B3), myo-inositol

• Amino acids and nitrogen sources: Glycine, glutamine, casein hydrolysate

• Carbohydrates: Sucrose (most common), glucose, maltose

• Plant Growth Regulators (PGRs): Auxins (IAA, IBA, NAA, 2,4-D), cytokinins (BAP, kinetin, zeatin), gibberellins, abscisic acid, ethylene inhibitors

• Solidifying agents: Agar, gellan gum, agarose

• Other additives: Activated charcoal, antibiotics, natural extracts (coconut water, banana powder)

• Water: Ultra-pure, tissue-culture grade

The composition and concentration of these components are tailored to the plant species, tissue type (explant), and the specific stage of culture (initiation, multiplication, rooting, etc.)

Types of Plant Tissue Culture Media

Applications of Plant Tissue Culture Media

Plant tissue culture media are essential for:

• Micropropagation: Rapid, large-scale multiplication of elite, disease-free plants

• Genetic Engineering: Transformation, gene editing, and regeneration of transgenic plants

• Germplasm Conservation: In vitro conservation of rare, endangered, or valuable plant species

• Production of Secondary Metabolites: Large-scale cell cultures for pharmaceuticals, flavors, and fragrances

• Pathogen Elimination: Meristem tip culture for virus-free stock in crops like potato, banana, and sugarcane

• Embryo Rescue and Hybridization: Culturing embryos from wide crosses or interspecific hybrids

• Research: Studying plant growth, development, stress tolerance, and physiology under controlled conditions

Key Stages and Media in Plant Tissue Culture

1. Initiation/Explant Establishment

   • Surface sterilization of explant (leaf, node, meristem, etc.)

   • Use of general or specialized initiation media (usually MS or LS base)

2. Callus Induction

   • High auxin/cytokinin ratio for callus formation (often 2,4-D or NAA plus BAP)

   • MS, B5, or SH media with specific PGRs

3. Organogenesis/Shoot Multiplication

   • Balanced or cytokinin-rich media (BAP, kinetin, zeatin)

   • MS, LS, or custom media for shoot proliferation

4. Rooting

   • Auxin-rich media (IBA, NAA, IAA)

   • MS or White’s medium, or specialized rooting blends (e.g., Roots™)

5. Acclimatization

   • Gradual transfer to soil or substrate, often with reduced sucrose and PGRs

Popular Plant Tissue Culture Media Products

Plant Tissue Culture Media Composition and Preparation

Essential Components

• Basal Salts: Provide macro- and micronutrients for cellular metabolism and growth

• Organic Supplements: Vitamins, amino acids, and sometimes natural extracts to support metabolism and morphogenesis

• Carbon Source: Sucrose is most common (20–30 g/L), but glucose or maltose may be used

• Plant Growth Regulators: Auxins for rooting/callus, cytokinins for shoot multiplication, gibberellins for elongation, abscisic acid for dormancy, and ethylene inhibitors as needed

• Gelling Agent: Agar (6–8 g/L), gellan gum, or agarose for solid media

• pH Adjustment: Typically 5.6–5.8 before sterilization

Preparation Steps

1. Weigh and dissolve basal salts in ~80% of final water volume (ultra-pure, tissue-culture grade)

2. Add organic supplements, vitamins, and PGRs; dissolve completely.

3. Adjust pH to 5.6–5.8 with 0.1–1M HCl or KOH

4. Add gelling agent (if making solid media); stir and heat gently.

5. Bring to final volume with water; mix thoroughly.

6. Dispense into culture vessels (tubes, jars, flasks, Petri dishes).

7. Sterilize by autoclaving at 121°C for 15–20 minutes at 15 psi

8. Cool and solidify in a sterile environment; store at 2–8°C if not used immediately.

How to Select the Right Plant Tissue Culture Media

• Start with standard media (MS, B5, LS, White’s) for most species

• Adjust salt concentration (high, medium, low) to optimize for your explant/species

• Test different ratios of auxin and cytokinin for shoot/root induction or callus formation

• Experiment with sucrose (2–6%) and other organic nutrients for optimal growth

• For woody plants or recalcitrant species, use specialized media (WPM, QL, DKW)

• For rooting, choose proprietary rooting media or auxin-rich blends (Roots™)

• For research or unique species, request custom formulations or consult technical support.

Plant Tissue Culture Media Kits and Ready-to-Use Solutions

• Starter Kits: Include MS or custom media, agar, PPM™ (for contamination control), culture vessels, and measuring tools—ideal for beginners and teaching labs

• Roots™ and Shoots™ Media: Proprietary blends for rooting and shoot multiplication, available in convenient sachets or bulk powder

• Custom Media: Tailored to your plant species, explant type, or experimental needs—available on request.

Quality Assurance and Regulatory Compliance

• Manufactured under cGMP and ISO standards for consistency and safety

• Batch-to-batch traceability with COA and MSDS for every lot.

• Internationally validated methods for research, commercial, and regulatory use.

• Animal-free and plant-based options available for sensitive applications.

Plant Tissue Culture Media in India

India is a global leader in plant tissue culture, with over 200 commercial labs and a production capacity exceeding 350 million plantlets per year10. Major crops include banana, potato, sugarcane, apple, pineapple, strawberry, gerbera, orchids, bamboo, and more. The National Certification System for Tissue Culture Raised Plants (NCS-TCP) ensures quality, disease-free, and true-to-type planting material for agriculture, horticulture, and forestry

Market Trends and Growth Drivers

• Rising demand for disease-free, high-yielding planting stock in agriculture and horticulture10.

• Biotechnology and pharma R&D fueling demand for specialized media.

• Expansion of commercial micropropagation for ornamentals, forestry, and fruit crops.

• Customization and innovation: New formulations for rapid multiplication, stress tolerance, and genetic transformation.

• Sustainability: Lower carbon and plastic footprint compared to traditional propagation.

Frequently Asked Questions (FAQ)

Q1: What is plant tissue culture media?
A: Plant tissue culture media are nutrient-rich blends supporting the aseptic growth of plant cells, tissues, or organs in vitro. They contain inorganic salts, vitamins, sugars, growth regulators, and gelling agents

Q2: What are the most commonly used media?
A: Murashige & Skoog (MS), Gamborg’s B5, Linsmaier & Skoog (LS), Nitsch & Nitsch (NN), White’s, Woody Plant Medium (WPM), and proprietary blends like Roots™ and Shoots™

Q3: How do I choose the right media for my plant species?
A: Start with standard media, then optimize salt, sugar, and PGR concentrations. For woody or recalcitrant species, use WPM or QL. Consult technical support for custom blends

Q4: What is the shelf life and storage condition for plant tissue culture media?
A: Most media powders last 2–5 years if stored cool, dry, and sealed. Prepared media should be used within weeks and stored at 2–8°C.

Q5: Are animal-free or plant-based media available?
A: Yes, animal-free and vegan media are available for sensitive or regulatory-compliant applications.

Q6: Can I buy plant tissue culture media in bulk or for export?
A: Yes, bulk packaging and export logistics are available for large-scale and international buyers.

Q7: What documentation is provided?
A: Every batch comes with a Certificate of Analysis (COA), Material Safety Data Sheet (MSDS), and full traceability.

Q8: How do I prepare and sterilize plant tissue culture media?
A: Dissolve powder in water, adjust pH, add gelling agent, autoclave at 121°C for 15–20 min, and pour into sterile vessels

Q9: What are the main applications of plant tissue culture media?
A: Micropropagation, genetic engineering, germplasm conservation, secondary metabolite production, pathogen elimination, research, and teaching

Q10: Do you provide technical support and custom formulations?
A: Yes, technical support and custom media blends are available for all research and commercial needs.

Buy Plant Tissue Culture Media in Various Packaging Options

Volume discounts are available for bulk orders. All packaging complies with UN and ISO safety regulations.

Buy Plant Tissue Culture Media for Research and Development

Plant tissue culture media are widely used in R&D for:

• Micropropagation and clonal propagation

• Genetic engineering and transformation

• Somatic embryogenesis and synthetic seed production

• Secondary metabolite and biopharmaceutical production

• Pathogen elimination and germplasm conservation

• Hybridization, embryo rescue, and polyploidy induction

• Teaching and academic research

Sustainability and Innovation

• Reduced carbon footprint: Lower CO₂ emissions compared to traditional propagation.

• Less plastic waste: Sustainable, recyclable packaging options.

• Long shelf life: Fewer replacements, less waste, and cost savings.

• Automated media preparation: Compatible with automated preparators for high-throughput labs.

For bulk orders, technical queries, or customized pricing, contact our support team at chemikalstore.com.