{"id":1595,"date":"2025-06-27T14:53:08","date_gmt":"2025-06-27T14:53:08","guid":{"rendered":"https:\/\/chemikalstore.com\/?post_type=product&p=1595"},"modified":"2026-03-31T06:25:54","modified_gmt":"2026-03-31T06:25:54","slug":"plant-tissue-culture-media-ingredients","status":"publish","type":"product","link":"https:\/\/chemikalstore.com\/index.php\/product\/plant-tissue-culture-media-ingredients\/","title":{"rendered":"Plant Tissue Culture Media Ingredients"},"content":{"rendered":"

Buy Plant Tissue Culture Media Ingredients Online \u2013 High-Purity Components for Micropropagation and Research<\/strong><\/h1>\n

The ingredients of plant tissue culture media are the basic building blocks to effective in vitro plant growth, and therefore, the high-quality elite plant varieties can be propagated with rapidity and disease-free conditions. By purchasing the ingredients of plant tissue culture media over the internet, you obtain ingredients of nutrient media of laboratory quality that are the basis of micropropagation, genetic engineering, germplasm conservation, and biopharmaceutical production. Our buy plant tissue culture media service is in India: we order, harvest, provide, and deliver high-quality, standardized ingredient with detailed documentation, reliable supply and in time.<\/p>\n

Plant Tissue Culture Media Ingredients \u2013 Overview and Scientific Significance<\/strong><\/h2>\n

The ingredients of the plant tissue culture media are carefully designed to mimic the nutritional and hormonal conditions within which the plant cell, tissue or organ would grow in vitro. These substances are macronutrients, micronutrients, vitamin, amino acids, carbohydrates, plant growth regulators (PGRs), gelling substances, and special additives. All of them are instrumental in cell division, morphogenesis and metabolic processes, and ingredient quality cannot be compromised to guarantee a set of reproducible results.<\/p>\n

Key Categories<\/strong><\/h2>\n
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\n\n\n\n\n\n\n\n\n\n\n\n\n
Category<\/strong><\/th>\nFunction<\/strong><\/th>\nExamples<\/strong><\/th>\n<\/tr>\n<\/thead>\n
Macronutrients<\/strong><\/td>\nProvide essential elements (N, P, K, Ca, Mg, S)<\/td>\nNH\u2084NO\u2083, KNO\u2083, CaCl\u2082, MgSO\u2084, KH\u2082PO\u2084<\/td>\n<\/tr>\n
Micronutrients<\/strong><\/td>\nSupply trace elements for enzyme function<\/td>\nFe-EDTA, MnSO\u2084, ZnSO\u2084, CuSO\u2084, CoCl\u2082, H\u2083BO\u2083<\/td>\n<\/tr>\n
Vitamins<\/strong><\/td>\nSupport metabolic pathways and coenzymes<\/td>\nThiamine (B1), Nicotinic Acid (B3), Pyridoxine (B6)<\/td>\n<\/tr>\n
Amino Acids<\/strong><\/td>\nAct as nitrogen sources and osmotic agents<\/td>\nGlycine, Glutamine, Casein Hydrolysate<\/td>\n<\/tr>\n
Carbohydrates<\/strong><\/td>\nProvide energy and carbon skeletons<\/td>\nSucrose, Glucose, Fructose<\/td>\n<\/tr>\n
Plant Growth Regulators<\/strong><\/td>\nControl cell division, organogenesis<\/td>\nAuxins (IAA, IBA, NAA), Cytokinins (BAP, Kinetin)<\/td>\n<\/tr>\n
Gelling Agents<\/strong><\/td>\nSolidify media for explant support<\/td>\nAgar, Gellan Gum, Agarose<\/td>\n<\/tr>\n
Organic Supplements<\/strong><\/td>\nEnhance growth in recalcitrant species<\/td>\nCoconut Water, Banana Powder, Activated Charcoal<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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Buy Plant Tissue Culture Media Ingredients \u2013 Detailed Component Analysis<\/strong><\/h2>\n

1. Macronutrients: The Structural Backbone<\/strong><\/h4>\n

Macronutrients are sources of nitrogen, phosphorus, potassium, calcium, magnesium and sulfur which are needed in millimolar amounts. They constitute the structure and functioning of the plant cells.<\/p>\n

Key Macronutrient Specifications<\/strong><\/h4>\n
\n
\n\n\n\n\n\n\n\n\n\n
Compound<\/strong><\/th>\nRole<\/strong><\/th>\nConcentration (mg\/L in MS Media)<\/strong><\/th>\n<\/tr>\n<\/thead>\n
Ammonium Nitrate (NH\u2084NO\u2083)<\/strong><\/td>\nPrimary nitrogen source<\/td>\n1,650<\/td>\n<\/tr>\n
Potassium Nitrate (KNO\u2083)<\/strong><\/td>\nProvides K\u207a and NO\u2083\u207b<\/td>\n1,900<\/td>\n<\/tr>\n
Calcium Chloride (CaCl\u2082)<\/strong><\/td>\nCell wall integrity, membrane function<\/td>\n440<\/td>\n<\/tr>\n
Magnesium Sulfate (MgSO\u2084)<\/strong><\/td>\nChlorophyll synthesis, enzyme activation<\/td>\n370<\/td>\n<\/tr>\n
Potassium Phosphate (KH\u2082PO\u2084)<\/strong><\/td>\nEnergy transfer, buffering<\/td>\n170<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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Deficiency Risks<\/strong>:<\/p>\n

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    Low nitrogen \u2192 Stunted growth, chlorosis<\/p>\n<\/li>\n

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    Low calcium \u2192 Shoot tip necrosis<\/p>\n<\/li>\n

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    Low magnesium \u2192 Leaf yellowing<\/p>\n<\/li>\n<\/ul>\n

    2. Micronutrients: Catalysts for Metabolic Activity<\/strong><\/h4>\n

    Micronutrients are needed in trace levels (micromolar concentrations) and are essential to enzyme activity, photosynthesis and redox reactions.<\/p>\n

    Key Micronutrient Specifications<\/strong><\/h4>\n
    \n
    \n\n\n\n\n\n\n\n\n\n
    Compound<\/strong><\/th>\nRole<\/strong><\/th>\nConcentration (mg\/L in MS Media)<\/strong><\/th>\n<\/tr>\n<\/thead>\n
    Fe-EDTA<\/strong><\/td>\nElectron transport, chlorophyll synthesis<\/td>\n36.7<\/td>\n<\/tr>\n
    Manganese Sulfate (MnSO\u2084)<\/strong><\/td>\nPhotosystem II activity<\/td>\n22.3<\/td>\n<\/tr>\n
    Zinc Sulfate (ZnSO\u2084)<\/strong><\/td>\nAuxin synthesis, enzyme cofactor<\/td>\n8.6<\/td>\n<\/tr>\n
    Boric Acid (H\u2083BO\u2083)<\/strong><\/td>\nCell wall formation, sugar transport<\/td>\n6.2<\/td>\n<\/tr>\n
    Copper Sulfate (CuSO\u2084)<\/strong><\/td>\nLignin synthesis, respiration<\/td>\n0.025<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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    Critical Insight<\/strong>: Iron is stabilized as Fe-EDTA to prevent precipitation at pH 5.8.<\/p>\n

    3. Vitamins and Amino Acids: Metabolic Enhancers<\/strong><\/h4>\n

    Vitamins act as coenzymes, while amino acids serve as organic nitrogen sources and osmoticum.<\/p>\n

    Essential Vitamins & Amino Acids<\/strong><\/h4>\n
    \n
    \n\n\n\n\n\n\n\n\n\n
    Component<\/strong><\/th>\nRole<\/strong><\/th>\nConcentration (mg\/L in MS Media)<\/strong><\/th>\n<\/tr>\n<\/thead>\n
    Thiamine (B1)<\/strong><\/td>\nCarbohydrate metabolism<\/td>\n0.1\u201310<\/td>\n<\/tr>\n
    Nicotinic Acid (B3)<\/strong><\/td>\nRedox reactions, NAD\/NADP synthesis<\/td>\n0.5\u20135<\/td>\n<\/tr>\n
    Pyridoxine (B6)<\/strong><\/td>\nAmino acid metabolism<\/td>\n0.5\u20135<\/td>\n<\/tr>\n
    Glycine<\/strong><\/td>\nPrecursor for purines, chlorophyll<\/td>\n2.0<\/td>\n<\/tr>\n
    Myo-Inositol<\/strong><\/td>\nMembrane formation, signal transduction<\/td>\n50\u2013100<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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    Pro Tip<\/strong>: Casein hydrolysate (500\u20132,000 mg\/L) provides organic nitrogen for challenging species like orchids.<\/p>\n

    4. Carbohydrates: Energy Substrates<\/strong><\/h4>\n

    The glucose and fructose used as energy come as a result of hydrolyzing sucrose (20-30 g\/L). They may be replaced with glucose (in case of monocots) or maltose (in case of embryogenesis).<\/p>\n

    5. Plant Growth Regulators (PGRs): Directors of Morphogenesis<\/strong><\/h4>\n

    PGRs are hormones which regulate cell division, organogenesis and rooting. The ratios preset developmental pathways.<\/p>\n

    PGR Applications by Stage<\/strong><\/h4>\n
    \n
    \n\n\n\n\n\n\n\n\n
    Stage<\/strong><\/th>\nAuxins<\/strong><\/th>\nCytokinins<\/strong><\/th>\nOutcome<\/strong><\/th>\n<\/tr>\n<\/thead>\n
    Callus Induction<\/strong><\/td>\n2,4-D (1\u20135 mg\/L)<\/td>\nLow\/Zero<\/td>\nUndifferentiated cell mass<\/td>\n<\/tr>\n
    Shoot Multiplication<\/strong><\/td>\nLow NAA (0.1\u20130.5 mg\/L)<\/td>\nBAP (1\u20133 mg\/L)<\/td>\n5\u201310x shoot proliferation<\/td>\n<\/tr>\n
    Rooting<\/strong><\/td>\nIBA (0.5\u20132 mg\/L)<\/td>\nZero<\/td>\nAdventitious root formation<\/td>\n<\/tr>\n
    Somatic Embryogenesis<\/strong><\/td>\n2,4-D + Kinetin<\/td>\nBalanced<\/td>\nEmbryo formation without fertilization<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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    Formulation Tip<\/strong>: Use NAA for stable auxin activity; IBA for root induction.<\/p>\n

    6. Gelling Agents and Additives<\/strong><\/h4>\n

    Gelling Agents<\/strong><\/h4>\n
    \n
    \n\n\n\n\n\n\n\n
    Agent<\/strong><\/th>\nConcentration<\/strong><\/th>\nAdvantages<\/strong><\/th>\nLimitations<\/strong><\/th>\n<\/tr>\n<\/thead>\n
    Agar<\/strong><\/td>\n6\u201310 g\/L<\/td>\nCost-effective, easy to use<\/td>\nMay contain impurities<\/td>\n<\/tr>\n
    Gellan Gum<\/strong><\/td>\n1.5\u20133 g\/L<\/td>\nClear, pure, low autoinhibition<\/td>\nRequires divalent cations (Ca\u00b2\u207a)<\/td>\n<\/tr>\n
    Agarose<\/strong><\/td>\n5\u20138 g\/L<\/td>\nHigh purity, ideal for protoplasts<\/td>\nExpensive<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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    Specialized Additives<\/strong><\/h4>\n
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      Activated Charcoal (0.5\u20132 g\/L)<\/strong>: Adsorbs phenolics and toxins.<\/p>\n<\/li>\n

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      Coconut Water (5\u201320% v\/v)<\/strong>: Natural source of cytokinins and nutrients.<\/p>\n<\/li>\n

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      Antibiotics (e.g., Timentin\u2122)<\/strong>: Suppress bacterial contamination.<\/p>\n<\/li>\n<\/ul>\n

      Popular Plant Tissue Culture Media Formulations<\/strong><\/h2>\n

      Standard Media Compositions<\/strong><\/h4>\n
      \n
      \n\n\n\n\n\n\n\n\n\n
      Medium<\/strong><\/th>\nBest For<\/strong><\/th>\nKey Features<\/strong><\/th>\n<\/tr>\n<\/thead>\n
      Murashige & Skoog (MS)<\/strong><\/td>\nUniversal for dicots and monocots<\/td>\nHigh nitrate, balanced micronutrients<\/td>\n<\/tr>\n
      Gamborg\u2019s B5<\/strong><\/td>\nLegumes, cell suspensions<\/td>\nLow NH\u2084\u207a, optimized for callus<\/td>\n<\/tr>\n
      Woody Plant (WPM)<\/strong><\/td>\nTrees (oak, pine, eucalyptus)<\/td>\nLow salts, high Ca\u00b2\u207a<\/td>\n<\/tr>\n
      Nitsch & Nitsch<\/strong><\/td>\nAnther\/pollen culture<\/td>\nHigh vitamins, biotin, folic acid<\/td>\n<\/tr>\n
      White\u2019s Medium<\/strong><\/td>\nRoot cultures, minimal growth<\/td>\nLow salt, high MgSO\u2084<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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      Preparation of Plant Tissue Culture Media: Step-by-Step Protocol<\/strong><\/h2>\n
        \n
      1. \n

        Weigh Components<\/strong>: The ingredients should be of analytical grade. Ready-made base salts (e.g. MS powder) are time-saving.<\/p>\n<\/li>\n

      2. \n

        Dissolve in Water<\/strong>: Add 800 mL of tissue-culture grade of water into a beaker. Stir with magnetic bar.<\/p>\n<\/li>\n

      3. \n

        Add Sequentially<\/strong>: Macronutrients \u2192 Micronutrients \u2192 Vitamins \u2192 Amino Acids \u2192 Sugars \u2192 PGRs<\/span><\/p>\n<\/li>\n

      4. \n

        Adjust pH<\/strong>: Use 0.1M KOH\/HCl to reach pH 5.8 (\u00b10.1).<\/p>\n<\/li>\n

      5. \n

        Add Gelling Agent<\/strong>: Sprinkle agar\/gellan gum slowly to avoid clumping.<\/p>\n<\/li>\n

      6. \n

        Autoclave<\/strong>: Sterilize at 121\u00b0C, 15 psi for 20 minutes.<\/p>\n<\/li>\n

      7. \n

        Pour Under Laminar Flow<\/strong>: Dispense 25\u201330 mL per Petri dish or culture vessel.<\/p>\n<\/li>\n<\/ol>\n

        Critical Checks<\/strong>:<\/h4>\n
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          Post-autoclave pH should be 5.6\u20135.8.<\/p>\n<\/li>\n

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          Media should solidify clearly without cloudiness.<\/p>\n<\/li>\n<\/ul>\n

          Quality Assurance and Storage Guidelines<\/strong><\/h2>\n
          \n
          \n\n\n\n\n\n\n\n\n\n
          Parameter<\/strong><\/th>\nRequirement<\/strong><\/th>\nRationale<\/strong><\/th>\n<\/tr>\n<\/thead>\n
          Purity<\/strong><\/td>\n\u226599% (ACS\/USP grade)<\/td>\nPrevents toxicity or inhibition<\/td>\n<\/tr>\n
          Water Content<\/strong><\/td>\n\u22640.1% (Karl Fischer)<\/td>\nEnsures accurate dosing<\/td>\n<\/tr>\n
          Heavy Metals<\/strong><\/td>\n\u226410 ppm (ICP-MS)<\/td>\nAvoids metabolic disruption<\/td>\n<\/tr>\n
          Storage<\/strong><\/td>\nCool (15\u201325\u00b0C), dry, dark environment<\/td>\nPrevents degradation and moisture uptake<\/td>\n<\/tr>\n
          Shelf Life<\/strong><\/td>\n24\u201336 months in sealed containers<\/td>\nMaintains chemical stability<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
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          Applications of Plant Tissue Culture Media Ingredients<\/strong><\/h2>\n
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            Micropropagation<\/strong>: Commercial generation of disease free banana, potato or orchids.<\/p>\n<\/li>\n

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            Genetic Engineering<\/strong>: CRISPR\/Cas9 editing in rice or tobacco.<\/p>\n<\/li>\n

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            Secondary Metabolite Production<\/strong>: Taxol from yew cell cultures.<\/p>\n<\/li>\n

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            Germplasm Conservation<\/strong>: Cryopreservation of endangered species.<\/p>\n<\/li>\n

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            Haploid Production<\/strong>: Doubled haploid technology in maize breeding.<\/p>\n<\/li>\n<\/ul>\n

            How to Buy Plant Tissue Culture Media Ingredients at chemikalstore.com<\/strong><\/h2>\n
              \n
            1. \n

              Visit chemikalstore.com<\/strong>\u00a0\u2192 Search “plant tissue culture media ingredients”.<\/p>\n<\/li>\n

            2. \n

              Filter by Application<\/strong>:<\/p>\n

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                Macronutrients<\/em>: NH\u2084NO\u2083, KNO\u2083, CaCl\u2082<\/p>\n<\/li>\n

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                PGRs<\/em>: IBA, BAP, 2,4-D<\/p>\n<\/li>\n

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                Gelling Agents<\/em>: Agar, Gellan Gum<\/p>\n<\/li>\n<\/ul>\n<\/li>\n

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                Review COA\/MSDS<\/strong>: Ensure batch-specific traceability.<\/p>\n<\/li>\n

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                Bulk Discounts<\/strong>: Orders >5 kg attract 10\u201315% discounts.<\/p>\n<\/li>\n

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                Express Shipping<\/strong>: Delivery across India in 3\u20137 business days.<\/p>\n<\/li>\n<\/ol>\n

                Frequently Asked Questions (FAQ)<\/strong><\/h2>\n

                Q1: Can I use food-grade agar for tissue culture?<\/strong>
                \nA: No. Food-grade agar contains impurities. Use tissue-culture-grade agar (e.g., HiMedia\u2122 TC Agar) for consistent results.<\/p>\n

                Q2: Why is my media turning yellow after autoclaving?<\/strong>
                \nA: Degradation of PGRs or caramelization of sucrose. Normalize pH to 5.8 and shorten the time of autoclave.<\/p>\n

                Q3: How do I store PGR stock solutions?<\/strong>
                \nA: Auxins\/Cytokinins: 1 mg\/mL in 0.1M NaOH\/DMSO at -20\u00b0C. Gibberellins: Ethanol stocks at 4\u00b0C.<\/p>\n

                Q4: What substitutes exist for coconut water?<\/strong>
                \nA: Banana powder (100mg\/L) or commercial cytokinin blends (e.g., 2iP).<\/p>\n

                Q5: Is sucrose mandatory? Can I use table sugar?<\/strong>
                \nA: Sucrose is optimal. Anti foaming agents that suppress growth are present in table sugar.<\/p>\n

                Q6: How to troubleshoot vitrification (hyperhydricity)?<\/strong>
                \nA: Decrease cytokinins, increase the concentration of gelling agent or add 0.3% of activated charcoal.<\/p>\n

                Q7: What\u2019s the shelf life of prepared media?<\/strong>
                \nA: 4\u20136 weeks at 4\u00b0C. Dispose of in case it is dry or dirty.<\/p>\n

                Q8: Can I reuse media components?<\/strong>
                \nA: Never reuse. The risks of cross-contamination are high.<\/p>\n

                Global Market Trends and Innovations<\/strong><\/h2>\n
                  \n
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                  Custom Media Kits Pre-weighed blends of species such as Cannabis or vanilla.<\/p>\n<\/li>\n

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                  Nanoparticle Delivery Nano-encapsulated PGRs to be able to release them.<\/p>\n<\/li>\n

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                  Automated Media Prep: Robotic systems for high-throughput labs.<\/p>\n<\/li>\n

                • \n

                  Sustainability: Seaweed or cassava biodegradable gelling agents Biodegradable gelling agents: full-size, technical enquiries or custom pricing would be handled by the customer care team at chemikalstore.com.<\/p>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"

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                    Laboratory-grade macronutrients, micronutrients, PGRs, and gelling agents<\/p>\n<\/li>\n

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                    Batch-specific COA, MSDS, and ISO 9001 certification<\/p>\n<\/li>\n

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                    Bulk packaging with volume discounts<\/p>\n<\/li>\n

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                    India-wide delivery in 3\u20137 days<\/p>\n<\/li>\n<\/ul>\n","protected":false},"featured_media":1657,"comment_status":"open","ping_status":"closed","template":"","meta":{"_bst_post_transparent":"default","_bst_post_title":"","_bst_post_layout":"default","_bst_post_sidebar_id":"","_bst_post_content_style":"default","_bst_post_vertical_padding":"default","_bst_post_feature":"","_bst_post_feature_position":"","_bst_post_header":false,"_bst_post_footer":false},"product_brand":[],"product_cat":[83,140],"product_tag":[210,211,212],"product_brands":[],"class_list":{"0":"post-1595","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-plant-tissue-culture","7":"product_cat-plant-tissue-culture-media-ingredients","8":"product_tag-plant-tissue-culture","9":"product_tag-plant-tissue-culture-media","10":"product_tag-plant-tissue-culture-media-ingredients","12":"first","13":"instock","14":"featured","15":"shipping-taxable","16":"product-type-simple","17":"entry","18":"content-bg","19":"loop-entry"},"_links":{"self":[{"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/product\/1595","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/comments?post=1595"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/media\/1657"}],"wp:attachment":[{"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/media?parent=1595"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=1595"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=1595"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=1595"},{"taxonomy":"product_brands","embeddable":true,"href":"https:\/\/chemikalstore.com\/index.php\/wp-json\/wp\/v2\/product_brands?post=1595"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}